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ferrochetelase fech  (GeneTex)
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GeneTex ferrochetelase fech
(A) Aconitase activity assay from control or FXN KO K562 cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592. Bar plots show mean ± SD. ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. (B) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for OXPHOS subunits or Tubulin. (C) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for Lipoic acid, which is conjugated to PDH-E2, KGDH-E2 and GCSH. Additional blots against OGDH (i.e. KGDH-E1) and Actin. (D) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against enzymes in the heme biosynthesis pathway- <t>FECH</t> and ALAD- or TOMM20. (E) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against POLD1, MMS19 or Actin. See also Figure S3.
Ferrochetelase Fech, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Aconitase activity assay from control or FXN KO K562 cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592. Bar plots show mean ± SD. ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. (B) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for OXPHOS subunits or Tubulin. (C) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for Lipoic acid, which is conjugated to PDH-E2, KGDH-E2 and GCSH. Additional blots against OGDH (i.e. KGDH-E1) and Actin. (D) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against enzymes in the heme biosynthesis pathway- FECH and ALAD- or TOMM20. (E) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against POLD1, MMS19 or Actin. See also Figure S3.

Journal: Cell

Article Title: Hypoxia rescues frataxin loss by restoring iron sulfur cluster biogenesis

doi: 10.1016/j.cell.2019.03.045

Figure Lengend Snippet: (A) Aconitase activity assay from control or FXN KO K562 cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592. Bar plots show mean ± SD. ****=p < 0.0001. One-way ANOVA with Bonferroni’s post-test. (B) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for OXPHOS subunits or Tubulin. (C) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted for Lipoic acid, which is conjugated to PDH-E2, KGDH-E2 and GCSH. Additional blots against OGDH (i.e. KGDH-E1) and Actin. (D) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against enzymes in the heme biosynthesis pathway- FECH and ALAD- or TOMM20. (E) Immunoblot of control or FXN KO cells grown in 21% O2, 1% O2 or 21% O2 with 75μM FG-4592, blotted against POLD1, MMS19 or Actin. See also Figure S3.

Article Snippet: Ferrochetelase (FECH) , GTX113435 , Genetex.

Techniques: Activity Assay, Control, Western Blot

Journal: Cell

Article Title: Hypoxia rescues frataxin loss by restoring iron sulfur cluster biogenesis

doi: 10.1016/j.cell.2019.03.045

Figure Lengend Snippet:

Article Snippet: Ferrochetelase (FECH) , GTX113435 , Genetex.

Techniques: